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It is a well-known fact that every human being differ from one another on various basis, such as capability, potential, personality, intelligence, achievement, aptitude, attitude and other physical features etc. due to hereditary and environmental factors. It is assumed that blood groups have genetic properties and may influence various personality traits. The present study examines the association of self-esteem of prospective teachers with their blood groups. The study was conducted on 350 prospective teachers of NCR (National Capital Region) Delhi. The results of one way ANOVA and t-test shows that there is a statistically significant difference in the self-esteem of teachers belonging to Blood Group ‘A’ and ‘O’ and Blood Group ‘B’ and ‘AB’ which were significant at 0.05 level whereas Blood Group ‘B’ and ‘O’ were significant at 0.01 level of significance.