A Comparative Evaluation Of The Efficacy Of Three Irrigant Activation Systems: Fanta Af Max File, Xp Endo Finisher File And Irri-Safe Ultrasonic Tip On Enterococcus Faecalis Eradication In Long Oval Canals Using Confocal Laser Scanning Microscope. "A Randomized In-Vitro Study"

Abstract

Purpose: To compare the efficacy of irrigant activation systems: Fanta AF Max, XP Endo finisher and Irri-safe ultrasonic tip on Enterococcus faecalis eradication and the maximum depth of the irrigant penetration into the dentinal tubules of long oval canals in single rooted teeth. Methods: Fifty-four extracted human single rooted teeth with oval canals were included. Teeth were prepared with Hyflex rotary system, the irrigation was done using 2.5% Sodium hypochlorite and 17% Ethylenediaminetetraacetic acid. After sterilization, the teeth were inoculated with Enterococcus faecalis for 21 days. The teeth were randomly allocated into three groups (n=14) according to the activation system used. The canals were irrigated using 5.25% Sodium hypochlorite then activated for 3 minutes. The teeth were splitted longitudinally, then treated by live /dead stains and examined using Confocal laser scanning microscope. Results: The XP Endo finisher file showed the highest percentage of bacterial death compared to the other groups where the difference was statistically significant regardless the root depths and levels (P-value <0.001). The results revealed that the coronal the middle parts showed the highest bacterial death percentage compared to the apical third with statistical significance (P-value<0.001) Regarding the irrigant penetration depth, the XP Endo finisher group showed that highest irrigant penetration depth values into the dentinal tubules when compared to the other groups. Conclusion: XP Endo Finisher had the highest ability to eradicate Enterococcus faecalis in addition, it showed the deepest irrigant penetration depths into the dentinal tubules as well.