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The aim of this study was to compare the efficacy of ultrasonic irrigation in combination with root conditioning on the exposure of collagen fibrils in extracted human teeth using a scanning electron microscope.
Thirty periodontally healthy teeth were used in this study which was root planed and sectioned longitudinally to get sixty specimens which were included in six groups containing ten specimens each. The groups included two control and four experimental groups. The control groups received saline and ultrasonic irrigation respectively. The experimental groups received citric acid and tetracycline hydrochloride conditioning with saline and ultrasonic irrigation respectively. These specimens were examined under a scanning electron microscope and visualized for presence of exposed collagen fibrils.
The results showed a significant amount of exposed collagen fibers in the group which underwent ultrasonic irrigation in combination with root conditioning..
It can be concluded that ultrasonic irrigation before and after root conditioning improves exposure of collagen fibrils, which may be desirable for clinical success in periodontal regenerative therapy.
The clinical significance of this current study is that when root planing is done, it leaves behind a smear layer which may be detrimental for periodontal reattachment. Root conditioning with chemicals removes the smear layer without exposing the collagen fibers on the dentinal surface. This study shows that ultrasonic irrigation in combination with root conditioning removes the smear layer and exposes the collagen fibers on the root surface which may be desirable in the success of outcome of regenerative or periodontal plastic surgery.