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Researchers persevered in their quest to find the effects of high-intensity training on physiological variables among university-level football players. We randomly selected thirty university-level football players from Chennai, Tamil Nadu, India, ranging from 17 to 23 years of age, in order to accomplish the purpose of this study. Each group consisted of 15 participants. As a control-group, bunch-2 did not participate in any special training after participating in their regular routine. Bunch-1 underwent high-intensity training for eight weeks and bunch-2 did not participate in any special training after their regular routine. The dependent variables were VO2 max and resting heart rate. The Harvard step test was used to assess Vo2 Max, and a digital pulse monitor was used to assess resting heart rate. Randomization was used both the pre- and post-tests. In this study, data were collected before and after a training period of eight weeks, and statistics were applied to the data collected to determine whether significant improvements in selected variables occurred from baseline to post-training. Researchers found that high-intensity training significantly improved physiological variables among university-level football players, including V2 max and resting heart rate.