Antimicrobial Efficacy of Three Different Disinfecting Systems against E.Faecalis A comparative- in vitro study

Abstract

Statement of the Problem: Disinfection is vital part of root canal treatment. Many of the studies have been published over the years  on root canal disinfection techniques but the results are  contradictory. Hence, this survey provides additional support to detect the most effective root canal disinfection protocol.

Purpose: The purpose of the survey is to determine the antimicrobial efficacy of photo-activated disinfection (PAD), diode laser irradiation, , endo-activator and conventional needle system with 2.5% sodium hypochlorite (NaOCl) on Enterococcus faecalis.

Materials and Method: With Pro Taper next file system, root canals of 60  freshly extracted premolar with single straight canals were prepared and later contaminated with E. faecalis suspension and incubated for 24hrs. All the samples were then randomly distributed into four groups which were irrigated with sodium hypochlorite and disinfected with all four disinfecting devices (Endoactivator, Diode laser, PAD, Conventional needle system).

Results: In the study,  a significant decline in the bacterial population after all treatments (P < 0.001) were observed. The laser and PAD irrigation were significantly more effective in reduction of bacterial loadi.e1.00 to 1.86x104 CFU/ml than endoactivator and conventional needle systemi.e2.86 to 5.23×104 CFU/ml.

Conclusion: The PAD and laser system were more successful in reducing the bacterial load from the root canal than the endoactivator and NaOCl syringe irrigation alone.