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Background: Endurance exercises such as walking, jogging, running, dancing etc., are considered beneficial for health, such as cardiovascular fitness, reduction in cardiovascular problems, hypertension, high cholesterol, joint and muscular pain, diabetes and strengthening the bones. Objective: the study aimed to estimate the key parameters of the liver among healthy volunteers of endurance exercise. Methods & Materials: Study participants were randomly selected from the University of the Punjab, Lahore, Pakistan. The participants were thus categorized into two groups, i.e. control group (CG) and the experimental group (EG). Each group was formed of fifty (50) subjects. A self-made exercise protocol of endurance exercise was applied to EG. Ethical approval was taken from the ethical research and review board of the University of the Punjab, Lahore, Pakistan. Informed consent was taken from every subject before participation in the study, and all subjects were informed about the risks and benefits of participation in the study. Five (05) ml blood sample was collected from all subjects. Each blood sample was marked with a different identification code. Results & Conclusions: The pre and post-test results were processed through the statistical package for social sciences (SPSS, version-26). On the basis of data analysis and findings the researcher concluded that endurance exercises such as walking, jogging and running on the biochemical parameters of liver.