Phenotypic And Molecular Detection Of Some Virulence Genes Among Clinical Isolates Of Pseudomonas Aeroginosa And P. Fluorescence

Abstract

The study was conducted for the period from September 2021 to March 2022, in Ghazi Hariri Hospital, Yarmouk, and the Medical City. The study included collecting 115 samples from inpatients and returning patients, males, and females, of different ages. The samples included (burns, wounds, and urine) to investigate the presence of Pseudomonas aeruginosa and Pseudomonas fluorescens and study its virulence factors phenotypically and at the molecular level, , as Pseudomonas aeruginosa (25) bacterial isolates were isolated from the total (115) isolates, with a percentage of (21.7%), which included (5) isolates from urine, (7) isolates from wounds, and (13) isolates from burns, which represented (20%), ( 28%), and (52%), respectively, from a total of 25 isolates. The results showed that 25 isolates in each of P. aeruginosa and P.flurosence gave positive isolates for the mexB gene, as the isolates of P. aeruginosa and P.flurosence bacteria isolated from the urine contained 25% and 20% of the MexB gene, respectively. , and by 25% and 60% of wounds, while from burns it was 50% and 20%, respectively. As for the PIcN gene, the study showed that the isolates of P. aeruginosa and P.flurosence bacteria isolated from the urine contained the PIcN gene by 18% and 16%. %, respectively, by 27% and 50% of wounds, while burns were 55% and 34%, respectively.

Also, the isolates of P. aeruginosa and P.flurosence bacteria isolated from the urine contained the LasB gene by 22% and 25%, respectively, and by 26% and 50%, respectively, from wounds, while the percentage of burn isolates reached 52% and 25%. As for the isolates of P. aeruginosa, P.flurosence isolated from the urine contained 20% and 19% of the AprA gene, respectively, and by 25% and 50%, respectively, from wounds, while the percentage of burn isolates reached 55% and 34%. Sequence of total bacterial isolates P. aeruginosa, P.flurosence