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Background: To assess alteration in blood alcohol concentration with time in samples collected from living human subjects
Materials & methods: A total of 10 volunteer subjects were enrolled in the study group. Thirty vacutainers, sodium fluoride, potassium oxalate, ten 10 ml syringes were kept ready. Eight subjects volunteer consumed alcohol commercial drink with alcohol concentration of 37.8 percent. Two volunteers did not consume any alcohol and was taken as control. The volume of alcohol consumed was not regulated. Half an hour after the last drink, 10 ml of venous blood was collected from each subject. Examination of sample was done on two intervals, day 1 and day 10 after collection. ‘Chemito GC 1000’ gas chromatograph with Flame ionization detector was used. Standard solution of ethanol was injected. Standard calibration curve was drawn for standard. All the samples were analysed and results were subjected to statistical analysis.
Results: The blood sample concentrations about subjects of study group and control group at day 1 was 15.6 mg% and 0 respectively. The blood sample concentrations about subjects of study group and control group at day 10 was 12.3 mg% and 0 respectively.
Conclusion: Immediate assessment of blood samples will help in getting accurate results and proper blood alcohol values.