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Practicum is an essential component of science learning at all levels of learning. However, in its implementation, there are still many who report the difficulties experienced by students. This study explores students' perceptions of the difficulty of practicum at the University. This research uses a quantitative approach. A total of 61 Science students were selected by sampling saturated with the criteria of having carried out animal structure practicum activities. The 30 items of the multilevel Likert scale statement were developed from three practicum indicators and six supporting factor indicators. The three indicators are preparation, implementation, and reporting. Respondents' responses were tabulated in Ms.Excel and analyzed with the Rasch model via Winstep 4.4.4. Students' perceptions of the difficulty of the practicum are good. It can be seen from the scheduled practicum activities and students' motivation to carry out the practicum well. However, in preparing the report, many students are still experiencing difficulties, and the practicum requires high concentration accuracy. Interestingly, men have better readiness to carry out practicum than women. Good time management and completeness of infrastructure need to be considered so that practicum activities can be carried out correctly and efficiently so that difficulties during practicum can be minimized.