High Performance Liquid Chromatography For The Simultaneous Estimation Of Anti-Ulcer Drugs In Pharmaceutical Dosage Form

Abstract

A simple, rapid, precise, accurate and sensitive reverse phase liquid chromatographic method has been developed for the simultaneous determination of Mosapride and Rabeprazole in bulk and pharmaceutical dosage form dosage form. The chromatographic method was standardized using Waters ODS (C18) RP Column, 250 mm x 4.6 mm. 5µm I.D column with UV detection at 255nm, Methanol: Ammonium Acetate buffer (pH=6) = 75:25and other conditions optimized were: flow rate (1.0 ml/minute), wavelength (255 nm), Run time was maintained at 8 minutes. The proposed method was successfully applied to the simultaneous determination of Mosapride and Rabeprazole in bulk and pharmaceutical dosage form. Optimized retention time of Mosapride and Rabeprazole is 2.069 and 2. 488.The method was linear over the range of 10µg/ml for Mosapride and Rabeprazole. The recovery was in the range of 98% to 102%. Linearity range was found to be 0-30 µg/ml for Mosapride and 0-60 µg/ml for Rabeprazole. The correlation coefficient was found to be 0.995. LOD was found to be 0.05mg/ml and LOQ was found to be 0.15mg/ml for Mosapride & The LOD was found to be 0.09mg/ml and LOQ was found to be 0.27mg/ml for Rabeprazole. The results of the forced degradation studies indicated the specificity of the developed method that has been developed. Different analytical performance parameters such as precision, accuracy, limit of detection, limit of quantification and robustness were determined according to International Conference on Harmonization (ICH) guidelines